Screening and Identification of Brain Pericyte-Selective Markers.

ABSTRACT:

Background

Pericytes, a type of mural cells, exert important functions in the CNS. One major challenge in pericyte research is the lack of pericyte-specific and subpopulation-specific markers.

Methods

To address this knowledge gap, we first generated a novel transgenic mouse line in which vascular smooth muscle cells (vSMCs) are permanently labeled with tdTomato. Next, we isolated PDGFRβ⁺tdTomato^- pericytes and PDGFRβ⁺tdTomato⁺ vSMCs from the brains of these mice and subsequently performed RNAseq analysis to identify pericyte-enriched genes.

Results

Using this approach, we successfully identified 40 pericyte-enriched genes and 158 vSMC-enriched genes, which are involved in different biological processes and molecular functio

SUBMITTER: Kang M 

PROVIDER: S-EPMC11799917 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature