Proximity Ligation Induced DNAzyme Motor Cooperating with Exponential Amplification Reaction for Accurate Low-Density Lipoprotein (LDL) Detection.

ABSTRACT: Low-density lipoprotein (LDL) is intricately associated with numerous clinical conditions, including dyslipidemia and metabolic-associated fatty liver disease (MAFLD), and its serum concentration is crucial for diagnostic purposes. However, the sensitive and accurate analysis of "intact" LDL is a significant difficulty, as conventional approaches typically focus solely on the detection of cholesterol or surface proteins of LDL. We developed a proximity ligation-induced DNAzyme motor that facilitates an outstanding amplification reaction for the precise and sensitive detection of LDL through the simultaneous recognition of the target ApoB and cholesterol. This technique facilitates the direct and accurate quantification of the concentration of "intact" LDL particles, as opposed to assessing

SUBMITTER: Ning L 

PROVIDER: S-EPMC11800028 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature