Isolation and optimization of Bacillus thuringiensis BRB-3 isolated from germinated black upland rice seeds for GABA production.

ABSTRACT: Gamma-aminobutyric acid (GABA) is a non-coded amino acid that recently gained increased attention within the functional food industry. This study aimed to isolate endophytic bacteria from sterilized surfaces of germinated black upland rice seeds for GABA production and optimization. A total of 38 isolates were screened for glutamic acid decarboxylase (GAD) activity using a colorimetric method. Among these, thirteen isolates exhibited positive GAD activity. The BRB-3 strain emerged as the most promising, producing the highest GABA levels (4.54 g/L) at an optimal pH of 6.5. However, the BRB-3 strain was sensitive to pH lower than 5.5; so the pH was adjusted to 6.5 through all experiments. Further identification of the BRB-3 strain was performed using 16S rRNA gene sequence, which placed it i

SUBMITTER: Wannasutta R 

PROVIDER: S-EPMC11800075 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature