Tuning Ion Current Rectifying Nanopipettes for Sensitive Detection of Methicillin-Resistant <i>Staphylococcus aureus</i>.

ABSTRACT: Infectious diseases pose a growing challenge in healthcare, with the increasing rate of antimicrobial resistance limiting therapeutic options available for treatment. Rapid detection of infections at the earliest opportunity can significantly improve patient outcomes. In this report, ion current rectifying quartz nanopipettes with ca. 109 nm orifices were utilized for the label-free detection of DNA indicative of methicillin-resistant Staphylococcus aureus (MRSA). By immobilizing probe DNA complementary to the mecA gene on the internal walls of the nanopipette, the detection was achieved by monitoring changes in ion current rectification (ICR) following probe-target hybridization. We demonstrate enhanced sensitivity by controlling the surface probe density, resulting in a tun

SUBMITTER: Denuga S 

PROVIDER: S-EPMC11800174 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature