Integrated Metabolomics and Proteomics Analysis of the Myocardium in a Mouse Model of Acute Viral Myocarditis.

ABSTRACT:

Background

Acute viral myocarditis (AVMC) is a common inflammatory disease affecting the myocardium and is often accompanied by severe metabolic disturbances. The molecular mechanisms underlying this disease are complex and not yet fully understood.

Methods and results

Coxsackievirus B3 (CVB3)-induced AVMC mouse models were established. By integrating ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS)-based metabolomics and data-independent acquisition (DIA)-based proteomics, we aimed to investigate the global influence of CVB3 infection on the myocardial metabolome and proteome in mice. Based on the criterion of OPLS-DA VIP > 1.0 and p value < 0.05, a total of 149 differential metabolites (DMs) were identified, including 64 upregulated and 8

SUBMITTER: Xue Y 

PROVIDER: S-EPMC11800238 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature