Impact of modulating leptin sensitivity on the transcriptomic profile of adult-derived hypothalamic mouse neurons.

ABSTRACT: The modulation of leptin sensitivity in hypothalamic neurons plays a crucial role in metabolic regulation and the development of obesity. Three distinct approaches, exposure to leptin (LEPA), administration of leptin antagonist (LANTA), and treatment with palmitate (PA), were explored in this study to assess their effects on adult-derived mHypoA-2/12 neurons and the resulting transcriptomic signatures. To this end, 3' mRNA-Seq transcriptome analysis was employed, unexpectedly revealing downregulation of key genes associated with the NOD-like receptor signaling pathway (Irf9, Mapk3, Stat2, Nfkbia, Ikbkg, Rela, Cxcl1, and Traf5), the C-type lectin receptor signaling pathway (Nfkb2, Irf9, Mapk3, Stat2, Nfkb

SUBMITTER: Oclon E 

PROVIDER: S-EPMC11800294 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature