Inducible pluripotent stem cells to study human mast cell trajectories.

ABSTRACT: Mast cells (MCs) are derived from CD34⁺ hematopoietic progenitors, consist of different subtypes, and are involved in several inflammatory conditions. However, our understanding of human MC developmental trajectories and subtypes has been limited by a scarcity of suitable cellular model systems. Herein, we developed an in vitro model of human MC differentiation from induced pluripotent stem cells (iPSC) to study human MC differentiation trajectories. Flow cytometry characterization of hemopoietic cells derived from the myeloid cells-forming complex (MCFC) revealed an initial increase in Lin^- CD34⁺ hematopoietic progenitors within Weeks 1-3, followed by an increase in CD34^- CD45RA^- SSC^low and SSC^high hem

SUBMITTER: Idelman G 

PROVIDER: S-EPMC11801248 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature