Quantitative label-free digital holographic imaging of cardiomyocyte optical volume, nucleation, and cell division.

ABSTRACT: Cardiac regeneration in newborn rodents depends on the ability of pre-existing cardiomyocytes to proliferate and divide. This capacity is lost within the first week of postnatal development when these cells rapidly switch from hyperplasia to hypertrophy, withdraw from the cell cycle, become binucleated, and increase in size. How these dynamic changes in cell size and nucleation impact cardiomyocyte proliferative potential is not well understood. In this study, we innovate the application of a commercially available digital holographic imaging microscope, the Holomonitor M4, to evaluate the proliferative responses of mononucleated and binucleated cardiomyocytes after CHIR99021 treatment, a model proliferative stimulus. This system enables long-term label-free quantitative tracking of primar

SUBMITTER: Huang H 

PROVIDER: S-EPMC11801258 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature