The first comprehensive study on isolation and genetic characterization of canine parvoviruses from dogs in Mizoram, India reveals the emergence of CPV-2c.

ABSTRACT:

Background

Canine parvovirus type-2 (CPV-2) is a highly contagious enteric pathogen of puppies with worldwide distribution.

Aims

Molecular epidemiology, genetic characterization, phylogenetic analysis, and isolation of the CPV-2 virus from clinically affected dogs in Mizoram, India over eight years.

Methods

A total of 202 samples (199 fecal samples, 2 vomita, and 1 tissue sample) were screened by PCR assay.

Results

103 out of 202 samples (50.99%) tested positive. Of the 103 positive samples, 83 samples were cloned and sequenced. Sequence analysis showed CPV-2c as the predominant variant (63.85%) followed by the 2a variant (26.5%), 2b (8.43%), and FPV (1.2%). Phylogenetic analyses of the CPV-2c sequences formed separate clusters and were ancestrally related to J

SUBMITTER: Chakraborty P 

PROVIDER: S-EPMC11801323 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature