Tuning a bioengineered hydrogel for studying astrocyte reactivity in glioblastoma.

ABSTRACT: Astrocytes play many essential roles in the central nervous system (CNS) and are altered significantly in disease. These reactive astrocytes contribute to neuroinflammation and disease progression in many pathologies, including glioblastoma (GB), an aggressive form of brain cancer. Current in vitro platforms do not allow for accurate modeling of reactive astrocytes. In this study, we sought to engineer a simple bioengineered hydrogel platform that would support the growth of primary human astrocytes and allow for accurate analysis of various reactive states. After validating this platform using morphological analysis and qPCR, we then used the platform to begin investigating how astrocytes respond to GB derived extracellular vesicles (EVs) and soluble factors (SF). These studies reveal tha

SUBMITTER: DePalma TJ 

PROVIDER: S-EPMC11801334 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature