Accelerating isotope dilution LC-MS-based desmosine quantification for estimating elastin turnover.

ABSTRACT:

Aims

Circulating total desmosine, representing endogenous systemic elastin degradation activity, is an emerging biomarker for mortality risk in several diseases and aging. However, the existing analytical method takes more than 23 hours to complete, limiting its potential applications. The objective of this study was to shorten the turnover time of a stable isotope dilution liquid chromatogram mass spectrometry-based desmosine assay.

Materials & methods

Plasma samples were analyzed using acid hydrolysis followed by solid-phase extraction and LC-MS. Two approaches to reduce assay time were tested: microwave-assisted acid hydrolysis and direct injection following solid-phase extraction.

Results

The combination of acid hydrolysis at 180°C for 8 minutes and a low-volume e

SUBMITTER: Kuzmanova E 

PROVIDER: S-EPMC11801354 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature