Saturated Transposon Analysis in Yeast as a one-step method to quantify the fitness effects of gene disruptions on a genome-wide scale.

ABSTRACT: Transposon insertion site sequencing (TIS) is a powerful tool that has significantly advanced our knowledge of functional genomics. For example, TIS has been used to identify essential genes of Saccharomyces cerevisiae, screen for antibiotic resistance genes in Klebsiella pneumoniae and determine the set of genes required for virulence of Mycobacterium tuberculosis. While providing valuable insights, these applications of TIS focus on (conditional) gene essentiality and neglect possibly interesting but subtle differences in the importance of genes for fitness. Notably, it has been demonstrated that data obtained from TIS experiments can be used for fitness quantification and the construction of genetic interaction maps, but this potential is only sporadically exploited. Here, we present a

SUBMITTER: Kingma E 

PROVIDER: S-EPMC11801604 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature