Uncovering the microbiota in renal cell carcinoma tissue using 16S rRNA gene sequencing.

ABSTRACT:

Introduction

Increasing evidence indicates an important role of microbiota in cancer development and progression, while little is known about the correlation between microbiota and renal cell carcinoma (RCC). Thus, we performed this study to profile the intratumoral microbiota possibly associated with RCC.

Materials and methods

Paired RCC and adjacent normal tissue samples were collected from 24 patients with RCC. V3-V4 variable region of microbial 16S rRNA gene was sequenced using Illumina MiSeq. Sequencing reads were processed using QIIME. Differentially abundant bacterial taxa between groups were identified by LEfSe, and their potential functions were inferred by PICRUSt.

Results

Decreased species diversity was presented in RCC tissues (Simpson index, P = 0.0340),

SUBMITTER: Wang J 

PROVIDER: S-EPMC11801973 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature