Expansion of induced pluripotent stem cells under consideration of bioengineering aspects: part 1.

ABSTRACT: To fully utilize the potential of human induced pluripotent stem cells (hiPSCs) for allogeneic stem cell-based therapies, efficient and scalable expansion procedures must be developed. For other adherent human cell types, the combination of microcarriers (MCs) and stirred tank bioreactors has been shown to meet these demands. In this study, a hiPSC quasi-perfusion expansion procedure based on MCs was developed at 100-mL scale in spinner flasks. Process development began by assessing various medium exchange strategies and MC coatings, indicating that the hiPSCs tolerated the gradual exchange of medium well when cultivated on Synthemax II-coated MCs. This procedure was therefore scaled-up to the 1.3-L Eppendorf BioBLU 1c stirred tank bioreactor by applying the lower limit of Zwietering's sus

SUBMITTER: Schneider SL 

PROVIDER: S-EPMC11802619 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature