Expansion of induced pluripotent stem cells under consideration of bioengineering aspects: part 2.

ABSTRACT: The manufacturing of allogeneic cell therapeutics based on human-induced pluripotent stem cells (hiPSCs) holds considerable potential to revolutionize the accessibility and affordability of modern healthcare. However, achieving the cell yields necessary to ensure robust production hinges on identifying suitable and scalable single-use (SU) bioreactor systems. While specific stirred SU bioreactor types have demonstrated proficiency in supporting hiPSC expansion at L-scale, others, notably instrumented SU multiplate and fixed-bed bioreactors, remain relatively unexplored. By characterizing these bioreactors using both computational fluid dynamics and experimental bioengineering methods, operating ranges were identified for the Xpansion^® 10 and Ascent™ 1 m² bioreactors i

SUBMITTER: Teale MA 

PROVIDER: S-EPMC11802622 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature