A transgenic mouse line with a 58 kb fragment deletion shows skeletal defects.

ABSTRACT: Previously, a transgenic mouse line lacking a 58 kb fragment deletion including the upstream and exon1 of Fam20a gene (58 kb deletion mouse) was generated and showed growth retardation. The aim of this study was to characterize the skeletal phenotype of the homozygous 58 kb deletion mouse (58 kb-/-). Our results showed that body size and bone length of the 58 kb-/- mice were smaller than those of wild-type (WT) mice. The microcomputed tomography (µCT) analyses of trabecular and cortical bones in the 58 kb-/- displayed lower bone volume, thinner trabeculae and thinner bone cortex as compared to WT. Histological examination of the 58 kb-/- growth plate demonstrated disorganized chondrocyte zones and extended hypertrophic zone. The qPCR results showed downregulation of several osteoblast diff

SUBMITTER: Alamoudi A 

PROVIDER: S-EPMC11802833 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature