Expanding Yarrowia lipolytica's metabolic potential for detoxification of cyanogenic glycosides in edible plants.

ABSTRACT: Cyanides are highly toxic chemicals in several edible plants that threaten food safety and human health. A phenotypically distinct Yarrowia lipolytica strain that efficiently detoxifies multiple cyanogenic glycosides from edible plants was constructed using a family 1 glycosyl-hydrolase (GH1). The strain displayed higher growth rates and metabolic activities when exposed to high concentrations of cyanides than the wild-type. It overexpressed genes that promoted the binding of molecular oxygen to the cytochrome iv complex. The engineered strain repressed fatty acid production to optimize energy production and activated the cyanide-resistant respiratory (AOX) pathway to circumvent HCN toxicity and maintain cellular homeostasis. It upregulated ribosome biogenesis, the sec-dependent protein ex

SUBMITTER: Azi F 

PROVIDER: S-EPMC11802928 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature