Integrating molecular markers and phenotypic analysis to assess cold tolerance in rice germplasm.

ABSTRACT: Rice (Oryza sativa L.) is a crucial staple food for most of the world's population. However, it is highly vulnerable to low temperatures, which can induce growth retardation and yield loss. In this study, we aimed to develop SNP- and Indel-based molecular markers for the key cold tolerance-related genes HAN1, COLD11, and COLD1. The HAN1 marker was designed using a KASP assay, which was effective for fluorescence-based detection, whereas COLD11 and COLD1 markers were gel electrophoresis-compatible, enabling easy application without complex equipment. Considering the polygenic nature of cold tolerance, we analyzed combined markers, which exhibited enhanced prediction accuracy compared to single-marker analysis. Based on these markers, we categ

SUBMITTER: Lee Y 

PROVIDER: S-EPMC11802937 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature