Shelf-Stable <i>Sporosarcina pasteurii</i> Formulation for Scalable Laboratory and Field-Based Production of Biocement.

ABSTRACT: Biocement is an environmentally friendly alternative to traditional cement that is produced via microbially induced calcium carbonate precipitation (MICP) and has great potential to mitigate the environmental harms of cement and concrete use. Current production requires on-site bacterial cultivation and the application of live culture to target materials, lacking the convenience of stable formulas that enable broad adoption and application by nonscientific professionals. Here, we report the development of a dry shelf-stable formulation of Sporosarcina pasteurii, the model organism for biocement production. At laboratory scale, when inoculated at an equivalent concentration of viable cells, we show that this formulation produces biocement equal in strength to that produced using live

SUBMITTER: Tuttle MJ 

PROVIDER: S-EPMC11803556 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature