Gene editing enables non-invasive in vivo PET imaging of human induced pluripotent stem cell-derived liver bud organoids.

ABSTRACT: Human induced pluripotent stem cell (hiPSC)-derived liver cell therapies such as hepatocyte-like cells and liver organoids could provide unlimited therapeutic cells for clinical transplantation, but an inadequate understanding of their in vivo fate impedes translation. Whole body in vivo imaging could enable monitoring of transplanted cell survival and/or expansion non-invasively over time, permitting robust comparisons between emerging therapies to identify those most effective. The human sodium iodide symporter (hNIS) is a radionuclide reporter gene facilitating whole body in vivo cell tracking by positron emission tomography (PET). We gene-edited a clinical Good Manufacturing Practice-compliant hiPSC line at the AAVS1 safe harbor locus enabling constitutive expressi

SUBMITTER: Ashmore-Harris C 

PROVIDER: S-EPMC11803834 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature