Optimizing longifolene production in Yarrowia lipolytica via metabolic and protein engineering.

ABSTRACT: Longifolene (C₁₅H₂₄) is a tricyclic sesquiterpene widely utilized in the cosmetics and fragrances due to its versatile applications. Traditional extraction methods from plants suffer from low titer and lengthy production cycles, while chemical synthesis is hampered by the compound's complex structure, leading to high costs and insufficient market supply. This study aimed to develop a microbial cell factory for enhanced longifolene production. The strategy involved integrating longifolene synthase from Pinus sylvestris (PsTPS) into Yarrowia lipolytica and employing multiple metabolic engineering approaches. Initially, key genes in the mevalonate (MVA) pathway were overexpressed to enhance longifolene precursor availability for longifolene biosynthe

SUBMITTER: Yao YT 

PROVIDER: S-EPMC11803839 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature