CENP-A/CENP-B uncoupling in the evolutionary reshuffling of centromeres in equids.

ABSTRACT:

Background

While CENP-A is the epigenetic determinant of the centromeric function, the role of CENP-B, a centromeric protein binding a specific DNA sequence, the CENP-B-box, remains elusive. In the few mammalian species analyzed so far, the CENP-B box is contained in the major satellite repeat that is present at all centromeres, with the exception of the Y chromosome. We previously demonstrated that, in the genus Equus, numerous centromeres lack any satellite repeat.

Results

In four Equus species, CENP-B is expressed but does not bind the majority of satellite-based centromeres, or the satellite-free ones, while it is localized at several ancestral, now-inactive, centromeres. Centromeres lacking CENP-B are functional and recruit normal amounts of CENP-A and CENP-C. The absen

SUBMITTER: Cappelletti E 

PROVIDER: S-EPMC11804003 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature