Exploring RNA cargo in extracellular vesicles for pleural mesothelioma detection.

ABSTRACT:

Background

Pleural Mesothelioma (PM) is a highly aggressive cancer, for which effective early detection remains a challenge due to limited screening options and low sensitivity of biomarkers discovered so far. While extracellular vesicles (EVs) have emerged as promising candidates for blood-based biomarkers, their role in PM has not been studied yet. In this study, we characterized the transcriptomic profile of EVs secreted by PM primary cells and explored their potential as a biomarker source for PM detection.

Methods

We collected cell culture supernatant from early-passage PM cell cultures derived from the pleural effusion of 4 PM patients. EVs were isolated from the supernatant using Qiagen exoEasy Maxi kit. RNA isolation from EVs was done using the mirVana PARIS kit. Fin

SUBMITTER: Kraft A 

PROVIDER: S-EPMC11804012 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature