Refining α-synuclein seed amplification assays to distinguish Parkinson's disease from multiple system atrophy.

ABSTRACT:

Background

Parkinson's disease (PD) and multiple system atrophy (MSA) are two distinct α-synucleinopathies traditionally differentiated through clinical symptoms. Early diagnosis of MSA is problematic, and seed amplification assays (SAAs), such as real-time quaking-induced conversion (RT-QuIC), offer the potential to distinguish these diseases through their underlying α-synuclein (α-Syn) pathology and proteoforms. Currently, SAAs provide a binary result, signifying either the presence or absence of α-Syn seeds. To enhance the diagnostic potential and biological relevance of these assays, there is a pressing need to incorporate quantification and stratification of α-Syn proteoform-specific aggregation kinetics into current SAA pipelines.

Methods

Optimal RT-QuIC assay conditio

SUBMITTER: Wiseman JA 

PROVIDER: S-EPMC11804046 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature