Clofarabine induces tumor cell apoptosis, GSDME-related pyroptosis, and CD8⁺ T-cell antitumor activity via the non-canonical P53/STING pathway.

ABSTRACT:

Background

Clofarabine (Clo) is a Food and Drug Administration (FDA)-approved drug for the treatment of acute lymphoblastic leukemia; however, its effects on solid tumors remain largely unknown.

Methods

In vitro and in vivo experiments have demonstrated the cytotoxic effects of Clo on melanoma and lung cancer. The molecular mechanisms of Clo-induced tumor cell death were analyzed using western blotting, ELISA, reverse transcription-PCR, immunofluorescence, co-immunoprecipitation (CO-IP), short hairpin RNA, co-culture, chromatin immunoprecipitation, and flow cytometry. Clinical data sets were used to analyze the correlation between stimulator of interferon genes (STING)-NFκB signaling and immune infiltration.

Results

In this study, Clo significantly reduced the growth

SUBMITTER: Wu J 

PROVIDER: S-EPMC11804206 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature