Positive sputum fungal culture, fungal sensitisation, and airway microbial diversity in asthmatic children.

ABSTRACT: Sensitisation to thermotolerant fungi such as Aspergillus fumigatus and Candida albicans, which can colonise the airways, is associated with poor lung function in children with asthma. Dysbiosis of bacteria and fungi in the airway microbiome has been reported between health and asthma but has yet to be characterised for fungal-sensitised asthmatic children. We investigated if microbial diversity of the airways is altered in fungal-sensitised school-age asthmatic children. Sputum samples from children with asthma who were fungal sensitised (n = 22) and non-fungal sensitised (n = 17) along with children without asthma (n = 15), aged 5-16 years were profiled by traditional microbiological culture, modified fungal culture, bacterial 16S, and fungal ITS2 next-generation sequencing. Microbiota w

SUBMITTER: Seear PJ 

PROVIDER: S-EPMC11804241 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature