Reelin Deficiency and Synaptic Impairment in the Adolescent Prefrontal Cortex Following Initial Synthetic Cannabinoid Exposure.

ABSTRACT:

Background

Adolescent cannabinoid exposure can have long-lasting effects on the brain, particularly in the prefrontal cortex, where the reelin protein plays a crucial role in neural organization. Chronic cannabinoid exposure leads to reelin deficiency and behavioral abnormalities, but the underlying mechanisms remain unclear. With the increasing use of synthetic cannabinoids (SCs) among young people, understanding these effects is crucial.

Methods

We examined the cellular and synaptic consequences of initial SC exposure in adolescent male mice 1 day after a single in vivo exposure to WIN 55,212-2. Our approach combined immunohistochemistry, Western blots, conditional CB₁ receptor (CB1R) knockout mouse lines, quantitative polymerase chain reaction, and ex vivo elec

SUBMITTER: Silva-Hurtado TJ 

PROVIDER: S-EPMC11804564 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature