Integrated and streamlined microfluidic device for molecular diagnosis of pathogen through direct PCR amplification.

ABSTRACT:

Background

Polymerase chain reaction (PCR) is a gold-standard method widely acknowledged for offering precise and rapid analysis of genetic material, particularly having a crucial role in detecting pathogens and diagnosing infectious diseases. However, the complexity of the PCR process (including nucleic acid extraction/purification, amplification, and amplicon detection) hinders its widespread adoption in point-of-care testing, where simplicity and rapidity are essential for practical use.

Results

In this study, we developed a microfluidic genetic analysis device that leverages direct PCR technology to simplify the entire PCR process, focusing on enhancing the usability and accessibility of the device for point-of-care diagnostics. Direct PCR, which enables direct DNA ampli

SUBMITTER: Lee D 

PROVIDER: S-EPMC11804585 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature