14-3-3 promotes sarcolemmal expression of cardiac Ca_V1.2 and nucleates isoproterenol-triggered channel superclustering.

ABSTRACT: The L-type Ca²⁺ channel (Ca_V1.2) is essential for cardiac excitation-contraction coupling. To contribute to the inward Ca²⁺ flux that drives Ca²⁺-induced-Ca²⁺-release, Ca_V1.2 channels must be expressed on the sarcolemma; thus the regulatory mechanisms that tune Ca_V1.2 expression to meet contractile demand are an emerging area of research. A ubiquitously expressed protein called 14-3-3 has been proposed to affect Ca²⁺ channel trafficking in nonmyocytes; however, whether 14-3-3 has similar effects on Ca_V1.2 in cardiomyocytes is unknown. 14-3-3 preferentially binds phospho-serine/threonine residues to affect many cellular processes and is known to regulate cardiac ion channels including Na_V

SUBMITTER: Spooner HC 

PROVIDER: S-EPMC11804677 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature