Cumulus cell expansion, nuclear maturation and embryonic development of bovine cumulus-oocyte complexes matured in varying concentrations of follicular fluid.

ABSTRACT: In this study, we tested the overall hypothesis that CC expansion and early embryo development would be improved by including follicular fluid (FF) from small or large follicles in the oocyte maturation medium. In the first experiment, FF aspirated from bovine abattoir ovaries was added to the maturation medium at 0, 25, 50, 75 or 100%. Images of individual COCs were captured at 0, 6, 12 and 19 hours (h) of the maturation period and analyzed to calculate change in the total area over time. Cumulus cell expansion was greatest in COCs matured in 75% and 50% FF, and these differences were detectable at 12 (75% FF only) and 19 h (50% and 75% FF) of maturation. The improvement in CC expansion was greatest when FF from small follicles was used. Treatments for the subsequent experiments were sele

SUBMITTER: Negron-Perez VM 

PROVIDER: S-EPMC11805436 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature