Droplet-based functional CRISPR screening of cell-cell interactions by SPEAC-seq.

ABSTRACT: Cell-cell interactions are essential for the function and contextual regulation of biological tissues. We present a platform for high-throughput microfluidics-supported genetic screening of functional regulators of cell-cell interactions. Systematic perturbation of encapsulated associated cells followed by sequencing (SPEAC-seq) combines genome-wide CRISPR libraries, cell coculture in droplets and microfluidic droplet sorting based on functional read-outs determined by fluorescent reporter circuits to enable the unbiased discovery of interaction regulators. This technique overcomes limitations of traditional methods for characterization of cell-cell communication, which require a priori knowledge of cellular interactions, are highly engineered and lack functional read-outs. As an example o

SUBMITTER: Faust Akl C 

PROVIDER: S-EPMC11805652 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature