Importance of the 5' untranslated region for recombinant enzyme production in isolated Bacillus subtilis 007.

ABSTRACT: The production of industrial enzymes requires an efficient expression system with a suitable host. This study investigated the isolated Bacillus subtilis 007 as a host for expressing three enzymes with potential application in the food industry. Firstly, testing the P_aprE and P₄₃ promoters and the corresponding 5' untranslated regions revealed great differences in the production of the recently discovered β-galactosidase from Paenibacillus wnnyii. Expression controlled by the P_aprE promoter yielded a significantly higher activity of 2515 µkat/L, compared to 56 µkat/L with the P₄₃ promoter. Modifications on the P_aprE core promoter region or the spacer, the sequence between the Shine-Dalgarno sequence and the start codon, did not improve

SUBMITTER: Senger J 

PROVIDER: S-EPMC11805744 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature