Fungal infection monitoring on corneal epithelium ex vivo model and its collection over polyethersulfone membrane for detecting Candida albicans and Aspergillus fumigatus.

ABSTRACT: In this paper we report a human ex vivo model of Aspergillus fumigatus and Candida albicans infection of the cornea. We demonstrate the ability to monitor fungal growth on the cornea through optical and immunofluorescence microscopy. Additionally, we establish a method for collection of fungal antigens in polyethersulfone (PES) membrane-based sample collectors from the surface of fungal inoculated corneas. Immunofluorescence microscopy was performed directly on both corneal tissue and PES membrane sample collectors. For the latter case, we devised an original ad-hoc method by attaching the membranes used for the collection on standard glass slides, which we call glass slide KIT. This enabled easy handling and improved efficiency for specifically recognizing the corresponding fungal infecti

SUBMITTER: Orgul S 

PROVIDER: S-EPMC11805772 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature