Unilocular adipocyte and lipid tracer for immunofluorescent images.

ABSTRACT: Adipose tissue is a highly dynamic endocrine organ that serves as the body's primary energy reservoir through the storage and mobilization of lipids. Adipocyte cellular size has been recognized as an indicator of cellular status; hypertrophic adipocytes are more prone to insulin resistance and the secretion of pro-inflammatory cytokines. Thus, the size and number of lipids is important to consider both in the clinic with a biopsy and when developing disease models and regenerative tissue constructs. Tools available to analyze adipocyte size are finely tuned for hematoxylin-eosin images and tend to be challenged by confocal derived z-stack images which contain intensity gradients. Therefore, ImageJ manual analysis is the commonly utilized tool to measure these images. With there being heter

SUBMITTER: Johnston EK 

PROVIDER: S-EPMC11805913 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature