Effects of hydrazone-based G-quadruplex ligands on <i>FANCJ/BRIP1</i>-depleted cancer cells and a <i>Caenorhabditis elegans dog-1^-/-</i> strain.

ABSTRACT: G-quadruplex (G4) DNAs are alternative nucleic acid structures, proposed to play important roles in regulating DNA replication, gene transcription, and translation. Several specialized DNA helicases are involved in cellular G4 metabolism, in some cases with redundant functions. Among them, human FANCJ/BRIP1, which has orthologs in all metazoans, is one of the most powerful G4 resolvases, believed to act mainly at DNA replication forks. Here, we tested the effects of a set of hydrazone-derivative G4 ligands in a FANCJ-knocked-out HeLa cell line and in a Caenorhabditis elegans strain, where DOG-1, a FANCJ ortholog, was disrupted, as a whole organism model system. Our results revealed that loss of FANCJ specifically sensitized cancer cells to FIM-15, a mono-guanylhydrazone deriv

SUBMITTER: Germoglio M 

PROVIDER: S-EPMC11806260 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature