Visualisation of Euglena gracilis organelles and cytoskeleton using expansion microscopy.

ABSTRACT: This article explores the use of expansion microscopy, a technique that enhances resolution in fluorescence microscopy, on the autotrophic protist Euglena gracilis A modified protocol was developed to preserve the cell structures during fixation. Using antibodies against key cytoskeletal and organelle markers, α-tubulin, β-ATPase, and Rubisco activase, the microtubular structures, mitochondria, and chloroplasts were visualised. The organisation of the cytoskeleton corresponded to the findings from electron microscopy while allowing for the visualisation of the flagellar pocket in its entirety and revealing previously unnoticed details. This study offered insights into the shape and development of mitochondria and chloroplasts under varying conditions, such as culture ages and light

SUBMITTER: Konupkova A 

PROVIDER: S-EPMC11806349 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature