Label-free (fluorescence-free) sensing of a single DNA molecule on DNA origami using a plasmon-enhanced WGM sensor.

ABSTRACT: The integration of DNA origami structures with opto-plasmonic whispering gallery mode (WGM) sensors offers a significant advancement in label-free biosensing, overcoming the limitations of traditional fluorescence-based techniques, and providing enhanced sensitivity and specificity for detecting DNA hybridization events. In this study, DNA origami acts as a scaffold for the precise assembly of plasmonic dimers, composed of gold nanorods (AuNRs), which amplify detection sensitivity by generating strong near-field enhancements in the nanogap between the nanorods. By leveraging the strong electromagnetic fields generated within the nanogap of the plasmonic dimer, this platform enables the detection of transient hybridization events between DNA docking strands and freely diffusing complementar

SUBMITTER: Ghamari S 

PROVIDER: S-EPMC11806501 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature