Exploring New Delhi Metallo Beta Lactamases in Klebsiella pneumoniae and Escherichia coli: genotypic vs. phenotypic insights.

ABSTRACT:

Background

Carbapenemase-producing Enterobacterales pose a serious clinical threat, particularly in high-burden settings of carbapenem-resistant Escherichia coli and Klebsiella pneumoniae (CREK), where rapid detection tools are essential to aid patient management. In this study, we focused on bla_NDM, the most frequently reported carbapenemase in the region, and evaluated a combined phenotypic (lateral flow) and genotypic (PCR and WGS) approach for its detection. This research underscores the utility of lateral flow assays as a practical alternative to resource-intensive genotypic methods, offering a scalable solution for settings with limited laboratory capacity.

Method

One hundred seventy-seven extensively drug-resistant strains were characterized using MALDI-TOF

SUBMITTER: Ain NU 

PROVIDER: S-EPMC11806598 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature