Vitrification affects the post-implantation development of mouse embryos by inducing DNA damage and epigenetic modifications.

ABSTRACT: Vitrification is widely used in assisted reproductive technology (ART) for female infertility, but the long-term effect on the embryo of vitrification has not been comprehensively studied. The study aimed to investigate the effect of vitrification on long-term development of mouse embryos. The warmed embryos which were frozen at 8-cell stage were cultured in vitro until the blastocyst stage and were transferred into recipients. Immunofluorescence staining was performed to evaluate the reactive oxygen species (ROS) level, mitochondrial function, cell apoptosis, DNA damage and histone epigenetic modification in blastocysts. Transmission electron microscopy (TEM) analysis was performed to exam the mitochondrial ultrastructure in blastocysts. The related gene expression and transcriptome profi

SUBMITTER: Chen Y 

PROVIDER: S-EPMC11806629 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature