Clinical diagnosis of Q fever by targeted next-generation sequencing for identification of Coxiella burnetii.

ABSTRACT:

Purpose

Q fever is a zoonotic bacterial disease caused by Coxiella burnetii. Due to its variable and non-specific clinical symptoms, the disease is often overlooked and underreported. To date, the identification of C. burnetii as the causative pathogen of Q fever using targeted next-generation sequencing (tNGS) has not been previously documented.

Methods

tNGS was performed on patients with acute fever of unknown etiology, and qPCR was confirmed for C. burnetii infection.

Results

tNGS was performed on 112 patients with acute fever of unknown etiology at Peking University Third Hospital between March 27 and September 20, 2024. C. burnetii was identified in blood samples from five patients, leading to a clinical diagnosis of Q fever. These diagnoses were subsequently con

SUBMITTER: Zhan S 

PROVIDER: S-EPMC11806902 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature