Characterization of Delta-7 Alkenone Desaturase in Haptophyte Gephyrocapsa huxleyi Through Heterologous Expression in Tisochrysis lutea.

ABSTRACT: The marine haptophyte Gephyrocapsa huxleyi is an ecologically and geochemically important phytoplankton due to its contribution to the global carbon cycle and its ability to biosynthesize certain alkenones. These alkenones are long-chain alkyl ketones with two to four trans-type double bonds. The genes encoding alkenone desaturase in G. huxleyi have not been experimentally characterized so far, partly due to the difficulty of inducing genetic transformation in G. huxleyi. Therefore, we introduced the putative alkenone delta-7 desaturase of G. huxleyi (designated "DesT") to the transformable and alkenone-producing haptophyte Tisochrysis lutea. We found two types of coding sequences for DesT, which are probably derived from the expression products of different alleles, and designated them "D

SUBMITTER: Yoneda K 

PROVIDER: S-EPMC11807052 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature