R-loops acted on by RNase H1 influence DNA replication timing and genome stability in Leishmania.

ABSTRACT: Genomes in eukaryotes normally undergo DNA replication in a choreographed temporal order, resulting in early and late replicating chromosome compartments. Leishmania, a human protozoan parasite, displays an unconventional DNA replication program in which the timing of DNA replication completion is chromosome size-dependent: larger chromosomes complete replication later then smaller ones. Here we show that both R-loops and RNase H1, a ribonuclease that resolves RNA-DNA hybrids, accumulate in Leishmania major chromosomes in a pattern that reflects their replication timing. Furthermore, we demonstrate that such differential organisation of R-loops, RNase H1 and DNA replication timing across the parasite's chromosomes correlates with size-dependent differences in chromatin accessibility, G qua

SUBMITTER: Damasceno JD 

PROVIDER: S-EPMC11807225 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature