The EUCAST Disk Diffusion Method for Antimicrobial Susceptibility Testing of Oral Anaerobes.

ABSTRACT: There is a need for standardized methods for antimicrobial susceptibility testing (AST) of anaerobic bacteria involved in oral and extra-oral infections. We tested the recently published EUCAST disk diffusion method for rapidly growing anaerobes on selected oral anaerobes. AST of 20 strains of Prevotella spp., 11 strains of Porphyromonas gingivalis, and six Fusobacterium nucleatum complex strains was performed with amoxicillin and metronidazole disks using EUCAST guidelines. Plates were incubated anaerobically, and inhibition zones were evaluated after 20 h (EUCAST recommendations) and again after 44 h. The recommended agar supported the growth of all 38 strains. Twenty-hour incubation was sufficient for the assessment of inhibition zone diameters of Fusobacterium strains. Although approve

SUBMITTER: Jensen AB 

PROVIDER: S-EPMC11807598 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature