Role of the FOXM1/CMA/ER stress axis in regulating the progression of nonalcoholic steatohepatitis.

ABSTRACT:

Background/aims

The molecular mechanisms driving nonalcoholic steatohepatitis (NASH) progression are poorly understood. This research examines the involvement of chaperone-mediated autophagy (CMA) in NASH progression.

Methods

Hepatic CMA activity was analysed in NASH mice and patients. Lysosome-associated membrane protein 2A (LAMP2A) was knocked down or overexpressed to assess the effects of hepatocyte-specific CMA on NASH progression. Mice received a high-fat diet or a methionine and choline-deficient diet to induce NASH. Palmitic acid was employed to mimic lipotoxicity-induced hepatocyte damage in vitro. The promoter activity of FOXM1 was evaluated via ChIP and dual-luciferase reporter assays.

Results

Hepatic CMA activity was substantially low in NASH mice and patie

SUBMITTER: Ma S 

PROVIDER: S-EPMC11807764 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature