Lyophilized human platelet lysate: manufacturing, quality control, and application.

ABSTRACT:

Background

A significant number of platelet concentrates (PCs) is discarded daily in blood banks due to limited shelf life. Human platelet lysate (HPL), derived from expired PCs, has gained attention as an ethical and sustainable cell culture media supplement in biomedical research and cell therapy production. However, HPL is subject to decisive disadvantages such as batch differences and lack of storage stability. To overcome these limitations and to enhance the applicability of HPL, we developed an HPL manufacturing protocol including a lyophilization process. The aim of this study was to investigate the influence of HPL lyophilization on parameters of quality control, including growth factor concentrations and the culture of human mesenchymal stromal cells (hMSCs).

Methods

SUBMITTER: Wendland K 

PROVIDER: S-EPMC11807961 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature