Modular cone-and-plate device for mechanofluidic assays in Transwell inserts.

ABSTRACT: In this work, we present a cost effective and open-source modular cone-and-plate (MoCAP) device that incorporates shear stress in the popular Transwell^® insert system. This system acts as a lid that incorporates flow into 24-well Transwell^® inserts while preserving the ability to conduct molecular profiling assays. Moreover, the MoCAP device can be rapidly reconfigured to test multiple shear stress profiles within a single device. To demonstrate the utility of the MoCAP, we conducted select assays on several different brain microvascular endothelial cell (BMEC) lines that comprise models of the blood-brain barrier (BBB), since shear stress can play an important role in BBB function. Our results characterize how shear stress modulates passive barrier function and GLUT1

SUBMITTER: Chavarria D 

PROVIDER: S-EPMC11807968 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature