Spatiotemporal transcriptomics elucidates the pathogenesis of fulminant viral myocarditis.

ABSTRACT: Fulminant myocarditis (FM) is a severe inflammatory condition of the myocardium that often results in sudden death, particularly in young individuals. In this study, we employed single-nucleus and spatial transcriptomics to perform a comprehensive analysis of coxsackievirus B3 (CVB3)-induced FM in A/J mice, spanning seven distinct time points pre- and post-treatment. Our findings reveal that mesothelial cells play a critical role in the early stage of myocarditis by acting as primary targets for CVB3 infection. This triggers the activation of macrophages, initiating a cascade of inflammation. Subsequently, pro-inflammatory Inflammatory_Mac and T cells infiltrate the myocardium, driving tissue damage. We also identified Cd8⁺ effector T cells as key mediators of cardiomyocyte inju

SUBMITTER: Li H 

PROVIDER: S-EPMC11808084 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature