Full-length inhibitor protein is the most effective to perturb human dUTPase activity.

ABSTRACT: It has been demonstrated recently that knockout of the dUTPase enzyme leads to early embryonic lethality in mice. However, to explore the physiological processes arising upon the lack of dUTPase an effective and selective enzyme inhibitor is much needed. A highly specific and strong binding proteinaceous human dUTPase inhibitor described by us recently was a promising starting point to develop a molecular tool to study temporal and conditional dUTPase inhibition in cellulo. Towards this end we determined the 3D crystal structure of the crystallizable amino terminal domain of inhibitor protein, named Stl^NT in complex with the human dUTPase and designed several point mutants based on the structure to improve the inhibition effectivity. The effect of Stl^NT and a peptide

SUBMITTER: Kohegyi B 

PROVIDER: S-EPMC11808092 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature