Unveiling immunogenic characteristics and neoantigens in endometrial cancer with POLE hotspot mutations for improved immunotherapy.

ABSTRACT:

Background

Immunotherapy, especially with the use of immune checkpoint inhibitors, has demonstrated efficacy for a variety of malignant tumors. However, the potential of immunotherapy for endometrial cancer (EC) with POLE mutations remains underexplored.

Methods

We utilized multiple databases and clinical specimens to investigate the immunogenicity profiles of EC patients carrying POLE mutations. One particular hotspot mutation POLE^P286R was identified and further studied. Consequently, by constructing human leukocyte antigen (HLA) tetramers and incubating them with patients' peripheral blood mononuclear cells (PBMCs), T cells capable of recognizing the POLE^P286R mutation were sorted for further transcriptomic, proteomic and T-cell receptor (TCR) sequen

SUBMITTER: Huang J 

PROVIDER: S-EPMC11808158 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature