Multispectral autofluorescence for label free classification of immune cell type and activation/polarization status.

ABSTRACT: Evaluating immune status is a challenging and time-consuming process that involves analysing various biomarkers through numerous assays. The sensitive label-free technique of multispectral imaging of cell autofluorescence involves directly assessing the molecular composition of cells to gather biological information. Cells were cultured in RPMI 1640 modified media supplemented with penicillin-streptomycin and 10% foetal bovine serum at 37°C, with 5% CO₂ and 95% humidity. Activation and differentiation was confirmed using immunofluorophores against relevant markers. Multispectral microscopy utilized defined spectral regions, which spanned the excitation (345-476 nm) and emission (414-675 nm) wavelength ranges. In total, 56 distinct spectral channels were applied. These channels c

SUBMITTER: Habibalahi A 

PROVIDER: S-EPMC11808199 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature